Journal
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
Volume 7, Issue 1, Pages 474-480Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.synbio.2021.12.002
Keywords
Laccase; Endocrine disrupting chemicals (EDCs)
Categories
Funding
- Macau Pui Ching Middle School
- Macau Science and Artificial Intelligence Education Association
- Macau Science and Technology Development Fund [FDCT0031/2019/PS]
- Macau Education and Youth Development Bureau
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Endocrine Disrupting Chemicals (EDCs) are molecules that can disrupt hormonal balance and cause reproductive system and health problems. Laccase-producing microorganisms have been identified as potential candidates for degrading EDCs. In this study, two recombinant laccases fused with a secretion peptide were created and expressed in Escherichia coli. The activity of the recombinant laccases in decolorizing a synthetic dye and degrading beta-estradiol was successfully demonstrated, indicating the potential of the laccase secretion system in degrading toxic compounds.
Endocrine Disrupting Chemicals (EDCs) are a group of molecules that can influence hormonal balance, causing disturbance of the reproductive system and other health problems. Despite the efforts to eliminate EDC in the environment, all current approaches are inefficient and expensive. In previous research, studies revealed that laccase-producing microorganisms may be a potential candidate for EDC degradation, as laccases have been found to be able to degrade many kinds of EDCs effectively and steadily. Here, we created two recombinant laccases, each fused with secretion peptide, Novel Signal Peptide 4 (NSP4), and expressed them in Escherichia coli (E. coli, BL21), together with one laccase without secretion peptide. We first optimized the culture condition of expressing these laccases. Then, we test the activity of the recombinant laccases of decolorizing of a synthetic dye, indigo carmine. Finally, we confirmed the secreted can degrade one of the EDCs, beta-estradiol, showing the potential of using the laccase secretion system to degrade toxic compounds.
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