Recent advances in immobilized ω-transaminase for chiral amine synthesis

Enzymatic synthesis of chiral amines has witnessed remarkable progress in the past decade. Prominently, using recombinant and evolved u-Transaminases (omega-TA), an ecofriendly and more efficient process was developed to replace well-established chemical processes for synthesizing chiral amines. However, the process employing a soluble form of enzyme needs additional downstream purification steps contributing to the total process cost. Thus, over the years, different immobilization approaches have been employed to streamline omega-TA downstream processes, widen the stability profile of TA in different mediums and conditions, alleviate product inhibition, and facilitate continuous flow operation. Herein, we summarize literature dedicated to immobilized omega-TA in chiral amine synthesis and present an overview of all the representative strategies used to improve the omega-TA process. We have discussed the immobilization matrices, cofactor and multi-enzyme immobilization strategies, one-pot immobilization, and purification of omega-TA for batch and continuous synthesis of optically active amines in different dedicated sections of the review. (c) 2022 Elsevier Ltd. All rights reserved.

Automated summary

This article summarizes the application of immobilized omega-TA in chiral amine synthesis and provides an overview of various strategies to improve the process.