Journal
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Volume 20, Issue 9, Pages 1737-1748Publisher
WILEY
DOI: 10.1111/jcmm.12880
Keywords
helium cold atmospheric plasma; platinum nanoparticles; antioxidants; apoptosis; reactive oxygen species
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Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan [25108503, 15H00892]
- Grants-in-Aid for Scientific Research [15H04904, 24108001, 25108503, 24108002, 15H00892] Funding Source: KAKEN
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Plasma is generated by ionizing gas molecules. Helium (He)-based cold atmospheric plasma (CAP) was generated using a high-voltage power supply with low-frequency excitation (60 Hz at 7 kV) and He flow at 2 l/min. Platinum nanoparticles (Pt-NPs) are potent antioxidants due to their unique ability to scavenge superoxides and peroxides. These features make them useful for the protection against oxidative stress-associated pathologies. Here, the effects of Pt-NPs on He-CAP-induced apoptosis and the underlying mechanism were examined in human lymphoma U937 cells. Apoptosis was measured after cells were exposed to He-CAP in the presence or absence of Pt-NPs. The effects of combined treatment were determined by observing the changes in intracellular reactive oxygen species (ROS) and both mitochondrial and Fas dependent pathway. The results indicate that Pt-NPs substantially scavenge He-CAP-induced superoxides and peroxides and inhibit all the pathways involved in apoptosis execution. This might be because of the SOD/catalase mimetic effects of Pt-NPs. These results showed that the Pt-NPs can induce He-CAP desensitization in human lymphoma U937 cells.
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