Journal
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
Volume 15, Issue -, Pages 4357-4377Publisher
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJGM.S355043
Keywords
circRNAs; ceRNAs; biomarker; colon adenocarcinoma; prognosis
Categories
Funding
- Innovation Project of Guangxi Graduate Education [YCBZ2021050]
- National Natural Science Foundation of China [81973533]
- Guangxi Science and Technology Project [AD19245197, 2019AC03004]
- 2019 Guangxi University High-level Innovation Team
- Project of Outstanding Scholars Program
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This study analyzed the differentially expressed circRNA in colon adenocarcinoma (COAD) and found that hsa_circ_0084927 is highly expressed and can be used as a diagnostic marker for COAD. The study also established a ceRNA network, which suggests that hsa_circ_0084927 may affect the prognosis of COAD through the regulation of cell cycle, apoptosis, and other pathways.
Object: This study aims to analyze the differentially expressed circRNA in colon adenocarcinoma (COAD) and evaluate its diagnostic and prognostic value. Analyze associated circRNA-miRNA-mRNA network in COAD. Methods and Materials: Real-time quantitative PCR (RT-PCR) was used to verify differentially expressed circRNA in COAD tissues and cells; Receiver operator characteristic (ROC) and Cox regression analysis were used to evaluating its diagnostic and prognostic value; Meanwhile we conducted CCK-8, invasion, and migration experiments in cell lines to explore the function of circRNA. In addition, a competitive endogenous RNA (ceRNA) network was established using bioinformatics methods to explore its prognostic value and potential functional mechanisms. Results: Our study found that hsa_circ_0084927 is highly expressed in COAD tissues and cell lines. Plasma hsa_circ_0084927 can be used as a diagnostic marker for COAD patients; hsacirc_0084927 can promote the proliferation, migration and invasion of COAD cells. In addition, we effectively constructed a ceRNA: network has_circ_0084927/miR-106b-5p/VEGFA. The ceRNA network indicates that hsacirc_0084927 may affect the prognosis of COAD through the regulation of cell cycle, apoptosis and other pathways. Conclusion: Our research results indicate that hsa_circ_0084927 has a cancer-promoting effect and may be used as a circulating tumor marker for COAD prognosis. In addition, this study proposes a new ceRNA network to provide new insights for the targeted therapy of COAD.
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