Journal
JOURNAL OF CELL SCIENCE
Volume 129, Issue 13, Pages 2673-2683Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.183103
Keywords
Chromatin; Real-time imaging; Single-domain antibody; Chromatin function; Epigenetic
Categories
Funding
- Agence Nationale de la Recherche (ANR) program [ANR-10-CESA-011]
- Universite de Toulouse 3 Paul Sabatier (Actions Transverses du Conseil Scientifique)
Ask authors/readers for more resources
Chromatin function is involved in many cellular processes, its visualization or modification being essential in many developmental or cellular studies. Here, we present the characterization of chromatibody, a chromatin-binding single-domain, and explore its use in living cells. This non-intercalating tool specifically binds the heterodimer of H2A-H2B histones and displays a versatile reactivity, specifically labeling chromatin from yeast to mammals. We show that this genetically encoded probe, when fused to fluorescent proteins, allows non-invasive real-time chromatin imaging. Chromatibody is a dynamic chromatin probe that can be modulated. Finally, chromatibody is an efficient tool to target an enzymatic activity to the nucleosome, such as the DNA damage-dependent H2A ubiquitylation, which can modify this epigenetic mark at the scale of the genome and result in DNA damage signaling and repair defects. Taken together, these results identify chromatibody as a universal non-invasive tool for either in vivo chromatin imaging or to manipulate the chromatin landscape.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available