Cotton and Flax Textiles Leachables Impact Differently Cutaneous Staphylococcus aureus and Staphylococcus epidermidis Biofilm Formation and Cytotoxicity

Bacteria can bind on clothes, but the impacts of textiles leachables on cutaneous bacteria remain unknown. Here, we studied for the first time the effects of cotton and flax obtained through classical and soft ecological agriculture on the representatives S. aureus and S. epidermidis bacteria of the cutaneous microbiota. Crude flax showed an inhibitory potential on S. epidermidis bacterial lawns whereas cotton had no effect. Textile fiber leachables were produced in bacterial culture media, and these extracts were tested on S. aureus and S. epidermidis. Bacterial growth was not impacted, but investigation by the crystal violet technique and confocal microscopy showed that all extracts affected biofilm formation by the two staphylococci species. An influence of cotton and flax culture conditions was clearly observed. Flax extracts had strong inhibitory impacts and induced the formation of mushroom-like defense structures by S. aureus. Conversely, production of biosurfactant by bacteria and their surface properties were not modified. Resistance to antibiotics also remained unchanged. All textile extracts, and particularly soft organic flax, showed strong inhibitory effects on S. aureus and S. epidermidis cytotoxicity on HaCaT keratinocytes. Analysis of flax leachables showed the presence of benzyl alcohol that could partly explain the effects of flax extracts.

Automated summary

This study investigated the effects of cotton and flax obtained through different agricultural methods on cutaneous bacteria. It was found that flax exhibited inhibitory effects on S. epidermidis bacteria, while cotton had no effect. Extracts of textile fibers were tested on bacteria and keratinocytes, and it was observed that all extracts affected biofilm formation by bacteria, but had no impact on resistance to antibiotics and biosurfactant production. Additionally, the extracts, especially those from organic flax, showed strong inhibitory effects on bacteria cytotoxicity. Analysis of flax extracts revealed the presence of benzyl alcohol, which could partly explain these effects.