Journal
JOURNAL OF CELL BIOLOGY
Volume 214, Issue 3, Pages 293-308Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201605090
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Funding
- National Institutes of Health, National Eye Institute [R01 EY015625]
- National Institute of Arthritis and Musculoskeletal and Skin Diseases [R01 AR048155, F32 AR062476]
- National Institute of General Medical Sciences [R01 GM108807]
- UK Medical Research Council [G0900113]
- Wellcome Trust [108429]
- Canadian Institutes of Health Research Fellowship
- Fondation pour la Recherche Medicale grant from Centre National de la Recherche Scientifique
- Institut National de la Sante et de la Recherche Medicale
- Institut Curie
- Fondation pour la Recherche Medicale [DEQ20140329491]
- CelTisPhyBio Labex [ANR-10-LBX-0038]
- IDEX Paris Sciences et Lettres Research University [ANR-10-IDEX-0001-02]
- MRC [G0900113] Funding Source: UKRI
- Medical Research Council [G0900113] Funding Source: researchfish
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Endomembrane organelle maturation requires cargo delivery via fusion with membrane transport intermediates and recycling of fusion factors to their sites of origin. Melanosomes and other lysosome-related organelles obtain cargoes from early endosomes, but the fusion machinery involved and its recycling pathway are unknown. Here, we show that the v-SNA RE VAMP7 mediates fusion of melanosomes with tubular transport carriers that also carry the cargo protein TYRP1 and that require BLOC-1 for their formation. Using live-cell imaging, we identify a pathway for VAMP7 recycling from melanosomes that employs distinct tubular carriers. The recycling carriers also harbor the VAMP7-binding scaffold protein VARP and the tissue-restricted Rab GTPase RAB38. Recycling carrier formation is dependent on the RAB38 exchange factor BLOC-3. Our data suggest that VAMP7 mediates fusion of BLOC-1-dependent transport carriers with melanosomes, illuminate SNARE recycling from melanosomes as a critical BLOC-3-dependent step, and likely explain the distinct hypopigmentation phenotypes associated with BLOC-1 and BLOC-3 deficiency in Hermansky-Pudlak syndrome variants.
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