Sensing Alzheimer's Disease Utilizing Au Electrode by Controlling Nanorestructuring

This paper reports the development of Alzheimer's disease (AD) sensor through early detection of amyloid-beta (A beta) (1-42) using simple nanorestructuring of Au sheet plate by oxidation-reduction cycle (ORC) via the electrochemical system. The topology of Au substrates was enhanced through the roughening and Au grains grown by a simple ORC technique in aqueous solutions containing 0.1 mol/L KCl electrolytes. The roughened substrate was then functionalized with the highly specific antibody beta-amyloid A beta (1-28) through HS-PEG-NHS modification, which enabled effective and direct detection of A beta (1-42) peptide. The efficacy of the ORC method had been exhibited in the polished Au surface by approximately 15% larger electro-active sites compared to the polished Au without ORC. The ORC polished structure demonstrated a rapid, accurate, precise, reproducible, and highly sensitive detection of A beta (1-42) peptide with a low detection limit of 10.4 fg/mL and a wide linear range of 10(-2) to 10(6 )pg/mL. The proposed structure had been proven to have potential as an early-stage Alzheimer's disease (AD) detection platform with low-cost fabrication and ease of operation.

Automated summary

This paper reports the development of an Alzheimer's disease sensor that can detect amyloid-beta (Aβ) (1-42) through simple nanorestructuring of a gold sheet plate. The sensor achieved rapid, accurate, precise, reproducible, and highly sensitive detection of Aβ (1-42) with a low detection limit and a wide linear range.