An Aptamer-Functionalised Schottky-Field Effect Transistor for the Detection of Proteins

The outbreak of the coronavirus disease 2019 (COVID-19) in December 2019 has highlighted the need for a flexible sensing system that can quickly and accurately determine the presence of biomarkers associated with the disease. This sensing system also needs to be easily adaptable to incorporate both novel diseases as well as changes in the existing ones. Here we report the feasibility of using a simple, low-cost silicon field-effect transistor functionalised with aptamers and designed to attach to the spike protein of SARS-CoV2. It is shown that a linear response can be obtained in a concentration range of 100 fM to 10 pM. Furthermore, by using a larger range of source-drain potentials compared with other FET based sensors, it is possible to look at a wider range of device parameters to optimise the response.

Automated summary

This study reports the feasibility of using a flexible sensing system, based on a silicon field-effect transistor, to rapidly and accurately detect biomarkers associated with COVID-19. The system is easily adaptable to new and existing diseases, and exhibits low cost and linear response characteristics.