Journal
JOURNAL OF CELL BIOLOGY
Volume 215, Issue 2, Pages 259-275Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201604095
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Funding
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior Progama de Doutorado-sanduiche no Exterior [99999.000424/2015-03]
- Human Frontier Science Program [CDA-00007/2011]
- German Research Foundation (DFG) [SFB 655]
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The arrangement of neurons into distinct layers is critical for neuronal connectivity and function. During development, most neurons move from their birthplace to the appropriate layer, where they polarize. However, kinetics and modes of many neuronal translocation events still await exploration. In this study, we investigate retinal ganglion cell (RGC) trans location across the embryonic zebrafish retina. After completing their translocation, RGCs establish the most basal retinal layer where they form the optic nerve. Using in toto light sheet microscopy, we show that somal translocation of RGCs is a fast and directed event. It depends on basal process attachment and stabilized microtubules. Interestingly, interference with somal translocation induces a switch to multipolar migration. This multipolar mode is less efficient but still leads to successful RGC layer formation. When both modes are inhibited though, RGCs fail to translocate and induce lamination defects. This indicates that correct RGC translocation is crucial for subsequent retinal lamination.
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