Citrus Peel Flavonoid Extracts: Health-Beneficial Bioactivities and Regulation of Intestinal Microecology in vitro

Citrus peel and its extracts are rich in flavonoids, which are beneficial to human health. In this study, the extraction, component analysis, biological activity and intestinal microbiota regulation of citrus peel flavonoid extracts (CPFEs) were investigated. CPFEs from 14 Chinese cultivars were purified by ultrasound-assisted extraction and XAD-16 macroporous resin. The total flavonoid content of lemon was greatest at 103.48 +/- 0.68 mg/g dry weight (DW) by NaNO2-Al(NO3)(3)-NaOH spectrophotometry. Using high-performance liquid chromatography-diode array detection, the highest concentrations of naringin, hesperidin and eriocitrin were found in grapefruit (52.03 +/- 0.51 mg/g DW), chachiensis (43.02 +/- 0.37 mg/g DW) and lemon (27.72 +/- 0.47 mg/g DW), respectively. Nobiletin was the most polymethoxylflavone in chachiensis at 16.91 +/- 0.14 mg/g DW. CPFEs from chachiensis and grapefruit had better antioxidant activity, alpha-glucosidase inhibitory and sodium glycocholate binding ability. In addition, chachiensis and grapefruit CPFEs had positive effects on intestinal microecology, as evidenced by a significant increase in the relative abundance of Bifidobacterium spp., and production of short-chain fatty acids, especially acetic acid, by a simulated human intestinal model. Collectively, our results highlight the biological function of CPFEs as prebiotic agents, indicating their potential use in food and biomedical applications.

Automated summary

This study investigated the extraction, component analysis, biological activity, and regulation of intestinal microbiota of citrus peel flavonoid extracts (CPFEs). The results showed that lemon had the highest total flavonoid content, while grapefruit, chachiensis, and lemon had the highest concentrations of naringin, hesperidin, and eriocitrin, respectively. CPFEs from grapefruit and chachiensis exhibited better antioxidant activity, alpha-glucosidase inhibitory activity, and sodium glycocholate binding ability. Furthermore, grapefruit and chachiensis CPFEs had positive effects on intestinal microecology, as they increased the relative abundance of Bifidobacterium spp. and the production of short-chain fatty acids.