Journal
FRONTIERS IN NUTRITION
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fnut.2022.868681
Keywords
skipjack tuna (Katsuwonus pelamis); dark muscle; angiotensin-I-converting enzyme (ACE) peptide; cytoprotective function; hypertension
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Funding
- National Natural Science Foundation of China [82073764]
- Ten-thousand Talents Plan of Zhejiang Province [2019R52026]
- Science and Technology Planning Project of Zhoushan of China [2019C21015]
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ACE inhibitory peptides with high activity were prepared from skipjack tuna dark muscle, and 14 novel ACEi peptides were identified. MWN, MEKS, MKKS, and LPRS exhibited significant ACE inhibitory activity.
To effectively utilize skipjack tuna (Katsuwonus pelamis) processing by-products to prepare peptides with high angiotensin-I-converting enzyme (ACE) inhibitory (ACEi) activity, Neutrase was selected from five kinds of protease for hydrolyzing skipjack tuna dark muscle, and its best hydrolysis conditions were optimized as enzyme dose of 1.6%, pH 6.7, and temperature of 50 degrees C using single factor and response surface experiments. Subsequently, 14 novel ACEi peptides were prepared from the high ACEi protein hydrolysate and identified as TE, AG, MWN, MEKS, VK, MQR, MKKS, VKRT, IPK, YNY, LPRS, FEK, IRR, and WERGE. MWN, MEKS, MKKS, and LPRS displayed significantly ACEi activity with IC50 values of 0.328 +/- 0.035, 0.527 +/- 0.030, 0.269 +/- 0.006, and 0.495 +/- 0.024 mg/mL, respectively. Furthermore, LPRS showed the highest increasing ability on nitric oxide (NO) production among four ACEi peptides combining the direct increase and reversing the negative influence of norepinephrine (NE), and MKKS showed the highest ability on directly decreasing and reversing the side effects of NE on the secretion level of endothelin-1 (ET-1) among four ACEi peptides. These findings demonstrate that seafood by-product proteins are potential ACEi peptide sources and prepared ACEi peptides from skipjack tuna dark muscle, which are beneficial components for functional food against hypertension and cardiovascular diseases.
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