Journal
JOURNAL OF CELL BIOLOGY
Volume 212, Issue 1, Pages 29-38Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201507122
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Funding
- Japan Society for the Promotion of Science
- Hori Sciences and Arts Foundation
- Toyoaki Scholarship Foundation
- Ono Medical Research Foundation
- MSD
- Grants-in-Aid for Scientific Research [15H05897, 15K08152, 15H02500] Funding Source: KAKEN
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Lipid droplets (LDs) in the nucleus of hepatocyte-derived cell lines were found to be associated with premyelocytic leukemia (PML) nuclear bodies (NBs) and type I nucleoplasmic reticulum (NR) or the extension of the inner nuclear membrane. Knockdown of PML isoform II (PML-II) caused a significant decrease in both nuclear LDs and type I NR, whereas overexpression of PML-II increased both. Notably, these effects were evident only in limited types of cells, in which a moderate number of nuclear LDs exist intrinsically, and PML-II was targeted not only at PML NBs, but also at the nuclear envelope, excluding lamins and SUN proteins. Knockdown of SUN proteins induced a significant increase in the type I NR and nuclear LDs, but these effects were cancelled by simultaneous knockdown of PML-II. Nuclear LDs harbored diacylglycerol O-acyltransferase 2 and CTP : phosphocholine cytidylyltransferase a and incorporated newly synthesized lipid esters. These results corroborated that PML-II plays a critical role in generating nuclear LDs in specific cell types.
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