Possible Ameliorative Effects of the Royal Jelly on Hepatotoxicity and Oxidative Stress Induced by Molybdenum Nanoparticles and/or Cadmium Chloride in Male Rats

Simple Summary The aim of the present investigation is valuable due to the importance of possible contaminations and negative effects of these cadmium chloride and molybdenum nanoparticles. The physicochemical properties of molybdenum nanoparticles have been characterized, as well as their ultrastructural organization. A rat experimental model was then employed to assess the liver toxicity of molybdenum nanoparticles, even in combination with CdCl2. The toxicity of molybdenum nanoparticles and cadmium chloride was estimated via liver damage by means of chemico-biological markers of liver injury, serum lipids, inflammation, oxidative status, and histological and immunohistochemistry patterns. Moreover, the possible effects of royal jelly were evaluated. The results clarified that both chemicals induced hepatic toxicity with the excessive triggering of reactive oxygen species that induced severe oxidative injury, histological alterations in the hepatic structure, and hepatic ultrastructure. These results are concurrent with obtaining normal biochemical levels in groups either treated with royal jelly or even a combination of royal jelly and two xenobiotics. The royal jelly is considered an essential potential source of natural antioxidants capable of frustrating the effects of oxidative injury, which is considered the main cause of many diseases. The present study aimed to investigate the effect of the royal jelly (RJ) on hepatotoxicity induced by molybdenum nanoparticles (MoO3-NPs), cadmium chloride (CdCl2), or their combination in male rats at biochemical, inflammation, immune response, histological, and ultrastructural levels. The physicochemical properties of MoO3-NPs have been characterized, as well as their ultrastructural organization. A rat experimental model was employed to assess the liver toxicity of MoO3-NPs, even in combination with CdCl2. Different cellular studies indicate divergent mechanisms, from increased reactive oxygen species production to antioxidative damage and cytoprotective activity. Seventy male rats were allocated to groups: (i) control; (ii) MoO3-NPs (500 mg/kg); (iii) CdCl2 (6.5 mg/kg); (iv) RJ (85 mg/kg diluted in saline); (v) MoO3-NPs followed by RJ (30 min after the MoO3-NPs dose); (vi) CdCl2 followed by RJ; and (vii) a combination of MoO3-NPs and CdCl2, followed by RJ, for a total of 30 successive days. Hepatic functions, lipid profile, inflammation marker (CRP), antioxidant biomarkers (SOD, CAT, GPx, and MDA), and genotoxicity were examined. Histological changes, an immunological marker for caspase-3, and transmission electron microscope variations in the liver were also investigated to indicate liver status. The results showed that RJ alleviated the hepatotoxicity of MoO3-NPs and/or CdCl2 by improving all hepatic vitality markers. In conclusion, the RJ was more potent and effective as an antioxidant over the oxidative damage induced by the combination of MoO3-NPs and CdCl2.

Automated summary

The aim of this study was to investigate the effects of royal jelly on hepatotoxicity induced by molybdenum nanoparticles, cadmium chloride, or their combination in male rats. The results showed that royal jelly alleviated the hepatotoxicity by improving all hepatic vitality markers and acted as a potent antioxidant against the oxidative damage induced by the combination of nanoparticles and cadmium chloride.