Journal
COMMUNICATIONS BIOLOGY
Volume 5, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s42003-022-03453-2
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Funding
- Canadian Institutes of Health Research [FDN154327, FDN159902]
- GlycoNET [ID-03]
- NIH [R01AI134895, R01AI077628, R01AI143916-01]
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This study determined the exact chemical structure of the biofilm matrix exopolysaccharide Pel using an improved isolation procedure and selective hydrolysis with the glycoside hydrolase PelA(h).
The genetic capacity to synthesize the biofilm matrix exopolysaccharide Pel is widespread among Gram-negative and Gram-positive bacteria. However, its exact chemical structure has been challenging to determine. Using a Pseudomonas aeruginosa strain engineered to overproduce Pel, improvements to the isolation procedure, and selective hydrolysis with the glycoside hydrolase PelA(h), we demonstrate that Pel is a partially de-N-acetylated linear polymer of alpha-1,4-N-acetylgalactosamine comprised predominantly of dimeric repeats of galactosamine and N-acetylgalactosamine.
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