4.7 Article

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells

Journal

ISCIENCE
Volume 25, Issue 5, Pages -

Publisher

CELL PRESS
DOI: 10.1016/j.isci.2022.104289

Keywords

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Funding

  1. K-CONNEX from the Japan Science and Technology Agency (JST), Core Center for iPS Cell Research from Japan Agency for Medical Research and Development (AMED) [JP21bm0104001]
  2. AMED-PRIME [21gm6410003h0001]
  3. Japanese Society for the Promotion of Science KAKENHI Grant (JSPS) [19K16104]
  4. iPS Cell Research Fund

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By combining transcriptome and proteome profiling, this study reveals the crucial role of posttranscriptionally regulated genes in the survival of iPSCs, especially those genes specifically necessary for iPSC survival.
The effects of transcription factors on the maintenance and differentiation of human-induced or embryonic pluripotent stem cells (iPSCs/ESCs) have been well studied. However, the importance of posttranscriptional regulatory mechanisms, which cause the quantitative dissociation of mRNA and protein expression, has not been explored in detail. Here, by combining transcriptome and proteome profiling, we identified 228 posttranscriptionally regulated genes with strict upregulation of the protein level in iPSCs/ESCs. Among them, we found 84 genes were vital for the survival of iPSCs and HDFs, including 20 genes that were specifically necessary for iPSC survival. These 20 proteins were upregulated only in iPSCs/ESCs and not in differentiated cells derived from the three germ layers. Although there are still unknown mechanisms that downregulate protein levels in HDFs, these results reveal that posttranscriptionally regulated genes have a crucial role in iPSC survival.

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