The chromosome-level genome for Toxicodendron vernicifluum provides crucial insights into Anacardiaceae evolution and urushiol biosynthesis

The lacquer tree (Toxicodendron vernicifluum (Stokes) F.A. Barkley) is an impor-tant tree with economic, industrial, and medicinal values. Here, we generated the reference genome of T. vernicifluum at the chromosome level with 491.93 Mb in size, in which 98.26% of the assembled contigs were anchored onto 15 pseudo -chromosomes with the scaffold N50 of 32.97 Mb. Comparative genomic analysis revealed the gene families related to urushiol biosynthesis were expanded, contributing to the ecological fitness and biological adaptability of the lacquer tree. We combined multi-omics data to identify genes that encode key enzymes in the T. vernicifluum urushiol and lignin biosynthetic pathways. Furthermore, the unique active metabolites, such as butin and fisetin, in cultivar lacquers were iden-tified by metabolism profiling. Our work would provide crucial insights into metabolite synthesis such as urushiol and lignin, meanwhile offer a basis for further exploration of the cultivation and breeding of T. vernicifluum and other Anacardiaceae members.

Automated summary

In this study, the reference genome of the lacquer tree was generated, revealing the expanded gene families associated with its biological adaptability. The identification of key enzyme genes and unique active metabolites in the lacquer tree using multi-omics data provides crucial insights into metabolite synthesis and lays the foundation for further exploration of its cultivation and breeding.