Journal
JOURNAL OF FUNGI
Volume 8, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/jof8040370
Keywords
Rhizoctonia solani AG1-IA; sheath blight; LC-MS; MS; virulent proteins; fungal proteomics
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Funding
- Indian Council of Agricultural Research (ICAR)
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The proteomic analysis of high virulent and less virulent isolates of Rhizoctonia solani revealed differentially expressed proteins related to fungal development, sporulation, pathogenicity, and other functions. The study also confirmed a positive correlation between the abundances of proteins and transcripts. This research provides insights into the pathogenicity mechanism of R. solani in causing sheath blight disease in rice.
Sheath blight of rice is a destructive disease that could be calamitous to rice cultivation. The significant objective of this study is to contemplate the proteomic analysis of the high virulent and less virulent isolate of Rhizoctonia solani using a quantitative LC-MS/MS-based proteomic approach to identify the differentially expressed proteins promoting higher virulence. Across several rice-growing regions in Odisha, Eastern India, 58 Rhizoctonia isolates were obtained. All the isolates varied in their pathogenicity. The isolate RS15 was found to be the most virulent and RS22 was identified as the least virulent. The PCR amplification confirmed that the RS15 and RS22 belonged to the Rhizoctonia subgroup of AG1-IA with a specific primer. The proteomic information generated has been deposited in the PRIDE database with PXD023430. The virulent isolate consisted of 48 differentially abundant proteins, out of which 27 proteins had higher abundance, while 21 proteins had lower abundance. The analyzed proteins acquired functionality in fungal development, sporulation, morphology, pathogenicity, detoxification, antifungal activity, essential metabolism and transcriptional activities, protein biosynthesis, glycolysis, phosphorylation and catalytic activities in fungi. A Quantitative Real-Time PCR (qRT-PCR) was used to validate changes in differentially expressed proteins at the mRNA level for selected genes. The abundances of proteins and transcripts were positively correlated. This study provides the role of the proteome in the pathogenicity of R. solani AG1-IA in rice and underpins the mechanism behind the pathogen's virulence in causing sheath blight disease.
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