Journal
BIOMEDICINES
Volume 10, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/biomedicines10040810
Keywords
oxidative stress; cytoskeleton; aquaporin; porcine; peritoneal dialysis
Categories
Funding
- Ministry of Science and Technology, Taiwan [108-2314-B-002-014, 109-2314-B-002-262]
- National Taiwan University [NTU-CCP-106R890606]
Ask authors/readers for more resources
Peritoneal dialysis is a renal replacement therapy, but long-term complications can compromise its effectiveness. Using a pig model, researchers found that disruption of the cell cytoskeleton could lead to peritoneal dysfunction and PD failure.
Being one of the renal replacement therapies, peritoneal dialysis (PD) maintains around 15% of end-stage kidney disease patients' lives; however, complications such as peritoneal fibrosis and ultrafiltration failure during long-term PD compromise its application. Previously, we established a sodium hypochlorite (NaClO)-induced peritoneal fibrosis porcine model, which helped to bridge the rodent model toward pre-clinical human peritoneal fibrosis research. In this study, the peritoneal equilibration test (PET) was established to evaluate instant functional changes in the peritoneum in the pig model. Similar to observations from long-term PD patients, increasing small solutes transport and loss of sodium sieving were observed. Mechanistic investigation from both in vivo and in vitro data suggested that disruption of cytoskeleton induced by excessive reactive oxygen species defected intracellular transport of aquaporin 1, this likely resulted in the disappearance of sodium sieving upon PET. Functional interference of aquaporin 1 on free water transport would result in PD failure in patients.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available