Fabrication of biogenic-silver nanoparticles functionalized electrospun membranes counteracting bacteria and enhance wound healing

Silver is a non-renewable precious metal with many applications in biomedicine. In this study, we reported fabrication of biogenic-silver nanoparticles (b-AgNPs) functionalized bactericidal membranes with wound healing (WH) activity by near filed electrospinning (NFES). Polycaprolactone (PCL) with or without polyethylene glycol (PEG) and(or) b-AgNPs were used as materials to fabricate membranes of different compositions and geometric shapes. Membranes were functionalized with b-AgNPs by using two methods; 1) combined dissolved, and 2) surface coating. The fabricated membranes were characterized by optical microscope, 2D/3D digital microscope, SEM, EDS, FTIR, and XRD. Water holding potential, water vapor transmission rate (WVTR), and membrane surface roughness were also characterized. Results revealed that the method of membrane functionalization with b-AgNPs and the addition of polyethylene glycol (PEG) has significantly affected bactericidal and WH activity of the membranes. The b-AgNPs functionalized membranes Mem-F and Mem-E showed bactericidal effects with 100% and 70% WH activity, respectively. The calcein-AM/PI staining of C2C12 cells, their adhesion and proliferation on the surface of MEM-F functionalized with b-AgNPs confirmed its noncytotoxic effects on C2C12 cells. Histological results showed that Mem-F has significantly enhanced cell proliferation and differentiation resulting in fast WH in SD-rats. In conclusion, b-AgNPs functionalized electrospun PCL membranes could be a potential candidate for their use as antibacterial as well as wound dressing material.

Automated summary

This study reported the fabrication of functionalized bactericidal membranes with wound healing activity using biogenic-silver nanoparticles (b-AgNPs) by near field electrospinning. The method of membrane functionalization with b-AgNPs and the addition of polyethylene glycol (PEG) significantly affected the bactericidal and wound healing activity of the membranes. The b-AgNPs functionalized membranes showed bactericidal effects and wound healing activity, making them potential candidates for antibacterial and wound dressing materials.