Journal
DIAGNOSTICS
Volume 12, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/diagnostics12051078
Keywords
interstitial cystitis/bladder pain syndrome; urothelium; lectin; glycosylation; mouse model; in vitro model
Categories
Funding
- Slovenian Research Agency (ARRS) [P3-0108, J3-2521]
Ask authors/readers for more resources
Studying changes in tissue glycosylation patterns may help discover biomarkers and therapeutic targets for IC/BPS. We compared glycosylation patterns of human urothelium between normal individuals and IC/BPS patients, as well as in experimental models of IC/BPS. Our results suggest potential biomarkers for histopathological diagnosis of IC/BPS and provide an adequate platform for preclinical study of IC/BPS glycobiology.
Pathophysiology of interstitial cystitis/bladder pain syndrome (IC/BPS) remains poorly understood, as well as its effective diagnosis and therapy. Studying changes in tissue glycosylation patterns under pathological conditions is a promising way of discovering novel biomarkers and therapeutic targets. The glycobiology of IC/BPS is largely understudied, therefore we compared glycosylation patterns of normal human urothelium with the urothelium of IC/BPS patients using a selection of 10 plant-based lectins with different monosaccharide preferences. We also compared lectin binding to human urothelium with the two most cited experimental models of IC/BPS, specifically, TNF alpha-treated human urothelial cell line RT4 and cyclophosphamide-induced chronic cystitis in C57BL6/J mice. Furthermore, binding of four of the selected lectins (ConA, DSL, Jacalin and WGA) was evaluated qualitatively by means of fluorescence microscopy, and quantitatively by fluorescence intensity (F.I.) measurements. Our results reveal a significant reduction in F.I. of Jacalin, as well as a prominent change in the WGA labeling pattern in the urothelium of IC/BPS patients, suggesting their potential use as promising additional biomarkers for histopathological diagnosis of IC/BPS. We have also shown that urothelial glycosylation patterns between selected experimental models and patients with IC/BPS are similar enough to offer an adequate platform for preclinical study of IC/BPS glycobiology.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available