QTL Mapping for Haploid Inducibility Using Genotyping by Sequencing in Maize

Doubled haploid (DH) technology in maize takes advantage of in vivo haploid induction (HI) triggered by pollination of donors of interest with inducer genotypes. However, the ability of different donors to be induced-inducibility (IND), varies among germplasm and the underlying molecular mechanisms are still unclear. In this study, the phenotypic variation for IND in a mapping population of temperate inbred lines was evaluated to identify regions in the maize genome associated with IND. A total of 247 F-2.3 families derived from a biparental cross of two elite inbred lines, A427 and CR1Ht, were grown in three different locations and Inclusive Composite Interval Mapping (ICIM) was used to identify quantitative trait loci (QTL) for IND. In total, four QTL were detected, explaining 37.4% of the phenotypic variance. No stable QTL was found across locations. The joint analysis revealed QTL x location interactions, suggesting minor QTL control IND, which are affected by the environment.

Automated summary

This study evaluated the phenotypic variation for inducibility (IND) in a mapping population of temperate inbred lines to identify regions in the maize genome associated with IND. In total, four QTL were detected, explaining 37.4% of the phenotypic variance. The joint analysis revealed QTL x location interactions, indicating that minor QTL control IND and are influenced by the environment.