Journal
PLANTS-BASEL
Volume 11, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/plants11091108
Keywords
faba bean; heat stress; sequencing; single nucleotide polymorphism; genome-wide association study; single trait; multi-trait
Categories
Funding
- CRP-grain legume program [100231]
- Arab Fund for Economic and Social Development (AFESD) for the operationalization of decentralized Center in five Arab countries [200072]
- China bilateral program [200230]
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Heat waves will become more frequent and intense, impacting global faba bean cultivation. The identification of molecular markers associated with heat tolerance can accelerate variety development. Through genome-wide association studies, several significant SNPs associated with heat tolerance were discovered.
Heat waves are expected to become more frequent and intense, which will impact faba bean cultivation globally. Conventional breeding methods are effective but take considerable time to achieve breeding goals, and, therefore, the identification of molecular markers associated with key genes controlling heat tolerance can facilitate and accelerate efficient variety development. We phenotyped 134 accessions in six open field experiments during summer seasons at Terbol, Lebanon, at Hudeiba, Sudan, and at Central Ferry, WA, USA from 2015 to 2018. These accessions were genotyped using genotyping by sequencing (GBS), and 10,794 high quality single nucleotide polymorphisms (SNPs) were discovered. These accessions were clustered in one diverse large group, although several discrete groups may exist surrounding it. Fifteen lines belonging to different botanical groups were identified as tolerant to heat. SNPs associated with heat tolerance using single-trait (ST) and multi-trait (MT) genome-wide association studies (GWASs) showed 9 and 11 significant associations, respectively. Through the annotation of the discovered significant SNPs, we found that SNPs from transcription factor helix-loop-helix bHLH143-like S-adenosylmethionine carrier, putative pentatricopeptide repeat-containing protein At5g08310, protein NLP8-like, and photosystem II reaction center PSB28 proteins are associated with heat tolerance.
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