Journal
FOODS
Volume 11, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/foods11091319
Keywords
aflatoxins B1; zearalenone; deoxynivalenol; monoclonal antibody; microfluidics; time-resolved fluorescence immunoassay; cereals
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Funding
- National Key Research and Development Program of China [2018YFC1602904]
- Guangdong Province Basic and Applied Basic Research Project [2018B030314005]
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This study developed a highly efficient time-resolved fluorescence immunoassay microfluidics method for sensitive detection of fungal toxins, especially AFB1, ZEN, and DON, in agricultural products.
The primary pollutants in cereal products are aflatoxins B1 (AFB1), zearalenone (ZEN), and deoxynivalenol (DON). In this study, anti-AFB1 MAb (4C9), anti-ZEN MAb (2A3), and anti-DON MAb (1F10) were developed and used in time-resolved fluorescence immunoassay microfluidics to determine AFB1, ZEN, and DON in agricultural products. The linear range for AFB1, ZEN, and DON were 0.05 similar to 2.2 mu g/kg, 1.45 similar to 375.75 mu g/kg, and 11.1 similar to 124.2 mu g/kg, respectively. In maize, the recoveries of AFB1/ZEN/DON were 92 similar to 101%, 102 similar to 105%, and 103-108%, respectively. High-performance liquid chromatography and the proposed approach had a good correlation. Time-resolved fluorescence immunoassay microfluidics is a highly efficient and sensitive field detection method for fungal toxins in agricultural products.
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