Journal
FOODS
Volume 11, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/foods11101450
Keywords
cow's milk; digested peptides; unique peptides; immunologic function
Categories
Funding
- National Natural Science Foundation of China [31760431]
- National Key R&D Program of China [2018YFC1604205]
- State Key Laboratory of Food Science and Technology, Nanchang University [SKLF-ZZA-201912]
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This study identified 109 BLG-derived peptides from simulated digestion of cow's milk, which showed good transport absorption capacity in Caco-2 cells. Some peptides were found to promote activation of DC and T cells.
Peptides, but not whole protein, elicit an allergic reaction since food allergens should be consumed by digestion. In this study, we explored the remaining peptides after simulated digestion of cow's milk in order to search for beta-lactoglobulin (BLG)-derived peptides that could play an immunomodulatory role. As a major allergen in milk, BLG-derived peptides, 109 in total, were identified both from simulated infant and adult digestion in vitro. These peptides were mainly located in four regions, and they were synthesized as five peptides, namely, BLG(1-14), BLG(24-35), BLG(40-60), BLG(82-101), and BLG(123-139). Then, the effect of peptides on the Caco-2 cell's transport absorption, the co-stimulatory molecules of DC, and the T-cell phenotype was explored. The results suggested all peptides showed better transport absorption capacity with the apparent permeability coefficient higher than 2 x 10(-6) cm.s(-1). The ability of BLG(40)(-60) for promoting lamina propria-derived DC cell (LPDC) maturation was observed by the increase in MHC II. Moreover, BLG(1)(-14) and BLG(40)(-60) directed activation of T lymphocytes towards a Th1 phenotype. This is the first report of the immunomodulatory potential of peptides in the sensitization of allergic reaction, and one peptide, BLG(40)(-60), was regarded as an immunomodulatory peptide, one that should be further explored in an animal model in depth.
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