Cold Thermal Priming of Laminaria digitata (Laminariales, Phaeophyceae) Gametophytes Enhances Gametogenesis and Thermal Performance of Sporophytes

Thermal characteristics of kelp species have been studied in many ways, but potentially persistent effects of temperature across generations are yet poorly understood. In this context, the effect of thermal priming on fertility and growth of the N-Atlantic kelp species Laminaria digitata was investigated within and across life cycle generations in a two-step common garden experiment. Using vegetative clonal gametophytes from cold (5 degrees C) and warm (15 degrees C) pre-experimental cultivation (3 years), we first quantified gametogenesis and recruitment over two weeks at a common temperature of 10 degrees C. Then, recruited sporophytes were transferred to a temperature gradient spanning the tolerance range of the species from 0 degrees C to 20 degrees C. We hypothesized that a warm gametophyte pre-experimental cultivation promotes performance of sporophytes at warm temperatures and vice versa. Interestingly, gametogenesis speed and sporophyte recruitment were higher in gametophytes following cold compared to warm pre-experimental cultivation, which indicates carry-over effects of temperature within the gametophyte generation. Compared to warm pre-experimental cultivation of gametophytes, a cold pre-experimental cultivation enhanced growth of juvenile Laminaria digitata sporophytes by more than 69% at the extreme low and high temperatures of 0 and 20 degrees C. This is the first evidence for a cross-generational effect between gametophyte parents and offspring sporophytes. As cold gametophyte cultivation increased the trait performance of gametogenesis, recruitment and thermal tolerance of juvenile sporophytes, priming of early life cycle stages may be used to increase resilience and productivity of kelps in marine forest restoration efforts and kelp mariculture.

Automated summary

This study investigates the effects of temperature on the fertility and growth of the kelp species Laminaria digitata. It finds that there are persistent effects of temperature on gametogenesis and recruitment within the gametophyte generation, and a cold pre-experimental cultivation enhances the growth and thermal tolerance of juvenile sporophytes. This cross-generational effect suggests that priming of early life cycle stages can increase the resilience and productivity of kelps in marine forest restoration and kelp mariculture.