4.5 Article

Fecal DNA Virome Is Associated with the Development of Colorectal Neoplasia in a Murine Model of Colorectal Cancer

Journal

PATHOGENS
Volume 11, Issue 4, Pages -

Publisher

MDPI
DOI: 10.3390/pathogens11040457

Keywords

bacteriophage; colorectal neoplasia; virome

Categories

Funding

  1. Australian Federal Government

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This study characterizes the alteration of the gut virome in azoxymethane-induced colorectal neoplasia in mice and identifies important viruses associated with tumor growth. The research shows that there is a significant shift in viral profile when tumors first appear, and certain viruses from the genera Brunovirus, Hpunavirus are positively associated with tumor growth, while viruses from Lubbockvirus show a negative correlation with tumor growth. These findings provide strong evidence of the potential role of bacteriophage in the development of colorectal neoplasia.
Alteration of the gut virome has been associated with colorectal cancer (CRC); however, when and how the alteration takes place has not been studied. Here, we employ a longitudinal study in mice to characterize the gut virome alteration in azoxymethane (AOM)-induced colorectal neoplasia and identify important viruses associated with tumor growth. The number and size of the tumors increased as the mice aged in the AOM treated group, as compared to the control group. Tumors were first observed in the AOM group at week 12. We observed a significantly lower alpha diversity and shift in viral profile when tumors first appeared. In addition, we identified novel viruses from the genera Brunovirus, Hpunavirus that are positively associated with tumor growth and enriched at a late time point in AOM group, whereas members from Lubbockvirus show a negative correlation with tumor growth. Moreover, network analysis revealed two clusters of viruses in the AOM virome, a group that is positively correlated with tumor growth and another that is negatively correlated with tumor growth, all of which are bacteriophages. Our findings suggest that the gut virome changes along with tumor formation and provides strong evidence of a potential role for bacteriophage in the development of colorectal neoplasia.

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