Journal
FRONTIERS IN MOLECULAR BIOSCIENCES
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmolb.2022.852911
Keywords
proximity-dependent biotinylation; BioID; APEX; post-translational modification; cellular organization; phosphorylation; mass spectrometry
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In recent years, proximity-dependent biotinylation approaches have been widely used to define the compositions of organelles and other structures. The contributions of post-translational modifications to protein compartmentalization, such as phosphorylation, have been discussed in this field.
In recent years, proximity-dependent biotinylation approaches, including BioID, APEX, and their derivatives, have been widely used to define the compositions of organelles and other structures in cultured cells and model organisms. The associations between specific proteins and given compartments are regulated by several post-translational modifications (PTMs); however, these effects have not been systematically investigated using proximity proteomics. Here, we discuss the progress made in this field and how proximity-dependent biotinylation strategies could elucidate the contributions of PTMs, such as phosphorylation, to the compartmentalization of proteins.
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