Journal
FRONTIERS IN MOLECULAR BIOSCIENCES
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmolb.2022.834020
Keywords
group II intron; RmInt1; IEP; ribonucleoprotein (RNP); ChIP-seq analysis
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In this study, we investigated the binding sites of RmInt1 in the Sinorhizobium meliloti genome using chromatin-immunoprecipitation coupled with next-generation sequencing. Our results showed that RmInt1 binding sites mainly cluster around the replication origin of each replicon in the S. meliloti genome. This study provides new evidence linking the mobility of group II introns to host DNA replication.
RmInt1 is a group II intron encoding a reverse transcriptase protein (IEP) lacking the C-terminal endonuclease domain. RmInt1 is an efficient mobile retroelement that predominantly reverse splices into the transient single-stranded DNA at the template for lagging strand DNA synthesis during host replication, a process facilitated by the interaction of the RmInt1 IEP with DnaN at the replication fork. It has been suggested that group II intron ribonucleoprotein particles bind DNA nonspecifically, and then scan for their correct target site. In this study, we investigated RmInt1 binding sites throughout the Sinorhizobium meliloti genome, by chromatin-immunoprecipitation coupled with next-generation sequencing. We found that RmInt1 binding sites cluster around the bidirectional replication origin of each of the three replicons comprising the S. meliloti genome. Our results provide new evidence linking group II intron mobility to host DNA replication.
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