4.6 Article

Evaluation of a Novel Chromogenic Medium for the Detection of Pseudomonas aeruginosa in Respiratory Samples from Patients with Cystic Fibrosis

Journal

MICROORGANISMS
Volume 10, Issue 5, Pages -

Publisher

MDPI
DOI: 10.3390/microorganisms10051004

Keywords

Pseudomonas aeruginosa; cystic fibrosis; culture media; chromogenic; aminopeptidase

Categories

Funding

  1. bioMerieux, France

Ask authors/readers for more resources

The study evaluated the culture of P. aeruginosa from respiratory samples of CF patients using Pseudomonas aeruginosa chromogenic agar (PACA), which proved to be a highly effective culture medium for the isolation and specific detection of P. aeruginosa from respiratory samples.
Pseudomonas aeruginosa is a dominant cause of respiratory infection in individuals with cystic fibrosis (CF), leading to significant morbidity and mortality. Detection of P. aeruginosa is conducted by culture of respiratory samples but this process may occasionally be compromised due to overgrowth by other bacteria and fungi. We aimed to evaluate a novel chromogenic medium, Pseudomonas aeruginosa chromogenic agar (PACA), for culture of P. aeruginosa from respiratory samples, from patients with CF. A total of 198 respiratory samples were cultured onto PACA and three other media: CHROMID (R) P. aeruginosa, CHROMagar (TM) Pseudomonas and MacConkey agar. P. aeruginosa was recovered from 66 samples (33%), using a combination of all media. After 72 h incubation, the sensitivity of the four chromogenic media was as follows: 91% for PACA and CHROMagar (TM) Pseudomonas, 85% for CHROMID (R) P. aeruginosa and 83% for MacConkey agar. For the three chromogenic media, the positive predictive value after 72 h was as follows: 95% for PACA, 56% for CHROMagar (TM) Pseudomonas and 86% for CHROMID (R) P. aeruginosa. PACA proved to be a highly effective culture medium for the isolation and specific detection of P. aeruginosa from respiratory samples.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available