Journal
MICROORGANISMS
Volume 10, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms10030527
Keywords
phosphorylcholine; Streptococcus pyogenes; PAF-R; emm genotype
Categories
Funding
- JSPS KAKENHI [20K18258]
- Grants-in-Aid for Scientific Research [20K18258] Funding Source: KAKEN
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This study investigates the role of phosphorylcholine (PC) in cell adhesion and invasion of Streptococcus pyogenes (S. pyogenes). The results show a negative correlation between PC expression level and bacterial adherence and invasion in S. pyogenes.
Phosphorylcholine (PC) is a structural component of various pathogens and is involved in bacterial adhesion via the platelet-activating factor receptor (PAF-R). In this study, we investigated how PC expression affects cell adhesion and invasion of Streptococcus pyogenes (S. pyogenes). Eight clinical strains of S. pyogenes were cultured, and PC expression was measured using fluorescence-activated cell sorting. Bacterial adherence and invasion were examined using Detroit 562 cells. An anti-PC-specific monoclonal antibody (TEPC-15) was used to inhibit bacterial PC, and a PAF-R antagonist (ABT-491) was used to inhibit cellular PAF-R. The emm gene was amplified by the polymerase chain reaction with the standard primers. The level of PC expressed on the S. pyogenes surfaces differed in each strain and differed even in the same emm genotype. Adherence assay experiments showed a significant negative correlation between TEPC-15 and ABT-491 inhibitory effects and PC expression in S. pyogenes. Similarly, intracellular invasion assay experiments showed a significant negative correlation between TEPC-15 and ABT-491 inhibitory effects and PC expression in S. pyogenes. This study suggests that S. pyogenes is involved in cell adhesion and invasion by PC.
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