Journal
MICROORGANISMS
Volume 10, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms10061134
Keywords
tick-borne pathogens; Ixodes ricinus; Nanopore sequencing; rickettsiales; Anaplasmataceae
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Funding
- Netherlands Organization for Health Research and Development (ZonMw) [52200-30-07]
- Dutch Ministry of Health, Welfare, and Sports
- NorthTick, European Union, European Regional Development Fund, in the North Sea Region Program
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This study presents the complete genomes of Ca. Neoehrlichia mikurensis derived from wildlife reservoir host tissues using advanced sequencing technologies. This approach offers an alternative to obtaining material from clinical cases and expensive bacterial isolation methods, expanding the potential for laboratory diagnostics. It also has implications for other difficult-to-culture tick-borne pathogens.
Ca. Neoehrlichia mikurensis is widely prevalent in I. ricinus across Europe and has been associated with human disease. However, diagnostic modalities are limited, and much is still unknown about its biology. Here, we present the first complete Ca. Neoehrlichia mikurensis genomes directly derived from wildlife reservoir host tissues, using both long- and short-read sequencing technologies. This pragmatic approach provides an alternative to obtaining sufficient material from clinical cases, a difficult task for emerging infectious diseases, and to expensive and challenging bacterial isolation and culture methods. Both genomes exhibit a larger chromosome than the currently available Ca. Neoehrlichia mikurensis genomes and expand the ability to find new targets for the development of supportive laboratory diagnostics in the future. Moreover, this method could be utilized for other tick-borne pathogens that are difficult to culture.
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