4.7 Article

Phytochemical Screening, Antioxidant and Antifungal Activities of Aconitum chasmanthum Stapf ex Holmes Wild Rhizome Extracts

Journal

ANTIOXIDANTS
Volume 11, Issue 6, Pages -

Publisher

MDPI
DOI: 10.3390/antiox11061052

Keywords

antioxidant assays; IC50 value; phytochemical analysis; antifungal activity; extraction; MIC value

Funding

  1. Vice Deanship of Research Chairs at King Saud University

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Aconitum chasmanthum Stapf ex Holmes, an essential and critically endangered medicinal plant from Kashmir Himalayas, was studied for its antioxidant and antifungal properties. The highest total phenolic and flavonoid content was found in the methanolic extracts. Methanolic extracts also showed the highest radical scavenging activities. Additionally, they exhibited potential inhibitory effects against Aspergillus niger, Aspergillus flavus, and Penicillium notatum.
Aconitum chasmanthum Stapf ex Holmes, an essential and critically endangered medicinal plant from Kashmir Himalayas, was studied for its antioxidant and antifungal properties. The shade-dried powdered rhizome was extracted sequentially with hexane, ethyl acetate, and methanol. These subsequent fractions were evaluated for total phenolic content (TPC); total flavonoid content (TFC); antioxidant assays, such as 1,1-diphenyl 1-2-picryl-hydrazyl (DPPH); ferric-reducing antioxidant power (FRAP); superoxide radical scavenging (SOR); hydroxyl radical scavenging (OH) and antifungal activity using the poisoned food technique. Highest TPC (5.26 +/- 0.01 mg/g) and TFC (2.92 +/- 0.04 mg/g) were reported from methanolic extracts. The highest values of radical scavenging activities were also observed in methanolic extracts with IC50 values of 163.71 +/- 2.69 mu g/mL in DPPH, 173.69 +/- 4.91 mu g/mL in SOR and 159.64 +/- 2.43 mu g/mL in OH. The chemical profile of ethyl acetate extract was tested using HR-LCMS. Methanolic extracts also showed a promising inhibition against Aspergillus niger (66.18 +/- 1.03), Aspergillus flavus (78.91 +/- 1.19) and Penicillium notatum (83.14 +/- 0.97) at a 15% culture filtrate concentration with minimum inhibitory concentration (MIC) values of 230 mu g/mL, 200 mu g/mL and 190 mu g/mL, respectively. Overall, the methanolic fractions showed significant biological potential, and its pure isolates might be used to construct a potential new medicinal source.

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