Journal
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2022.842530
Keywords
strontium-substituted calcium silicate; silk fibroin; bone marrow-derived mesenchymal stem cells; osteogenesis; angiogenesis; bone regeneration
Funding
- National Natural Science Foundation of China [82071082, 81771038, 81871490]
- Science and Technology Commission of Shanghai Municipality [19142202200]
- Young Doctor Collaborative Innovation Team of Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine [QC 2018-02, jyyq08201621]
- Excellent Youth Program of Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine [QC 2018-02, jyyq08201621]
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Strontium-substituted calcium silicate/silk fibroin composite materials have significant effects on the proliferation, osteogenic differentiation, and angiogenic factor secretion of rat bone marrow-derived mesenchymal stromal cells (rBMSCs), and can promote new bone formation in a rat calvarial defect model.
Achieving rapid osteogenesis and angiogenesis was the key factor for bone regeneration. In the present study, the strontium-substituted calcium silicate (SrCS)/silk fibroin (SF) composite materials have been constructed by combining the different functional component ratios of SrCS (12.5 wt%, 25 wt%) and SF. Then, the effects of SrCS/SF materials on proliferation, osteogenic differentiation, and angiogenic factor secretion of rat bone marrow-derived mesenchymal stromal cells (rBMSCs) were first evaluated in vitro. Moreover, the in vivo effect of osteogenesis was evaluated in a critical-sized rat calvarial defect model. In vitro studies showed that SrCS/SF significantly enhanced the cell proliferation, alkaline phosphatase (ALP) activity, and the expression of osteogenic and angiogenic factors of rBMSCs as compared with the SF and CS/SF, and the optimum proportion ratio was 25 wt%. Besides, the results also showed that CS/SF achieved enhanced effects on rBMSCs as compared with SF. The in vivo results showed that 25 wt% SrCS/SF could obviously promote new bone formation more than SF and CS/SF. The present study revealed that SrCS could significantly promote the osteogenic and angiogenic activities of SF, and SrCS/SF might be a good scaffold material for bone regeneration.
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