Journal
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2022.877603
Keywords
COVID19; SARS-cov-2; lateral flow assay; RT-LAMP; rapid diagnostics; point of care diagnostics; multiplexed loop mediated isothermal amplification
Funding
- Office of the Director of National Intelligence (ODNI), Intelligence Advanced Research Projects Activity (IARPA) [2020-20090400002]
- National Science Foundation [ECCS-1542148]
Ask authors/readers for more resources
This study demonstrates a rapid, scalable, high-throughput multiplex diagnostic method capable of detecting multiple gene targets of the SARS-CoV-2 virus in non-laboratory settings.
The global pandemic caused by the SARS-CoV-2 virus has underscored the need for rapid, simple, scalable, and high-throughput multiplex diagnostics in non-laboratory settings. Here we demonstrate a multiplex reverse-transcription loop-mediated isothermal amplification (RT-LAMP) coupled with a gold nanoparticle-based lateral flow immunoassay (LFIA) capable of detecting up to three unique viral gene targets in 15 min. RT-LAMP primers associated with three separate gene targets from the SARS-CoV-2 virus (Orf1ab, Envelope, and Nucleocapsid) were added to a one-pot mix. A colorimetric change from red to yellow occurs in the presence of a positive sample. Positive samples are run through a LFIA to achieve specificity on a multiplex three-test line paper assay. Positive results are indicated by a characteristic crimson line. The device is almost fully automated and is deployable in any community setting with a power source.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available