Journal
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2022.826694
Keywords
droplet magnetofluidics; point-of-care diagnostics; sample preparation; lysis; miniaturized rapid PCR; Candida auris; multidrug resistance; fungal pathogen
Funding
- Sherrilyn and Ken Fisher Center for Environmental Infectious Diseases at Johns Hopkins University [FCDP-010ZHA2020]
- National Institutes of Health [R01AI137272, R01AI138978, R61AI154628]
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Candida auris is an emerging multidrug-resistant fungal pathogen that has spread widely. Researchers have developed a POC device based on Droplet magnetofluidics technology to quickly detect the presence of C. auris.
Candida auris is an emerging multidrug-resistant fungal pathogen that can cause severe and deadly infections. To date, C. auris has spurred outbreaks in healthcare settings in thirty-three countries across five continents. To control and potentially prevent its spread, there is an urgent need for point-of-care (POC) diagnostics that can rapidly screen patients, close patient contacts, and surveil environmental sources. Droplet magnetofluidics (DM), which leverages nucleic acid-binding magnetic beads for realizing POC-amenable nucleic acid detection platforms, offers a promising solution. Herein, we report the first DM device-coined POC.auris-for POC detection of C. auris. As part of POC.auris, we have incorporated a handheld cell lysis module that lyses C. auris cells with 2 min hands-on time. Subsequently, within the palm-sized and automated DM device, C. auris and control DNA are magnetically extracted and purified by a motorized magnetic arm and finally amplified via a duplex real-time quantitative PCR assay by a miniaturized rapid PCR module and a miniaturized fluorescence detector-all in <= 30 min. For demonstration, we use POC.auris to detect C. auris isolates from 3 major clades, with no cross reactivity against other Candida species and a limit of detection of similar to 300 colony forming units per mL. Taken together, POC.auris presents a potentially useful tool for combating C. auris.
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