Journal
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2022.854130
Keywords
BAC; Cunninghamia lanceolata (lamb; ); genome; Chinese fir; China
Funding
- Seed industry innovation and industrialization project of Fujian Province [ZYCX-LY-202101]
- Youth Foundation of the Natural Science Foundation of Jiangsu Province [BK20210614]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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In this study, a bacterial artificial chromosome (BAC) library of Chinese fir was constructed and sequenced, providing insights into the genome structure of Chinese fir and generating gene data that will facilitate molecular investigations on tree growth mechanisms.
Cunninghamia lanceolata (Lamb.)Hook is an important economic timber tree in China. However, its genome characteristics have not been extensively assessed. To better understand its genome information, the bacterial artificial chromosome (BAC) library of chinese fir was constructed. A total of 422 BAC clones were selected and divided into 10 pools and sequenced, and with an average insert size of 121 kb, ranging from 97 to 145 kb. A total of 61,902,523 bp of reference sequences were sequenced and assembled, and based on an estimated genome size of 11.6 Gb for Chinese fir, the BAC library was estimated to have a total coverage of 0.53% genome equivalents. Bioinformatics analyses were also performed for repeated sequences, tRNAs, coding gene prediction, and functional annotation. The results of this study provide insights into the brief structure of the Chinese fir genome and has generated gene data that will facilitate molecular investigations on the mechanisms underlying tree growth.
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