4.7 Article

Uncovering Regulators of Heterochromatin Mediated Silencing Using a Zebrafish Transgenic Reporter

Journal

Publisher

FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2022.832461

Keywords

heterochromatin; zebrafish; NSD1; transgene

Funding

  1. National Institute of General Medical Sciences of the National Institutes of Health [R01GM110092, R35GM139556]
  2. NIH [T32GM007103]

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This study demonstrates a new in vivo tool for monitoring heterochromatin-mediated repression of repetitive sequences in vertebrate development and reveals a novel role for zebrafish NSD1 orthologs in heterochromatin-mediated repression.
Heterochromatin formation and maintenance is critical for the repression of transcription from repetitive sequences. However, in vivo tools for monitoring heterochromatin mediated repression of repeats in the context of vertebrate development have been lacking. Here we demonstrate that a large concatemeric transgene integration containing the dsRed fluorescent reporter under the control of a ubiquitous promoter recapitulates molecular hallmarks of heterochromatic silencing, and that expression from the transgene array can be reactivated by depletion of known regulators of heterochromatin. We then use this reporter to identify a previously unappreciated role for the zebrafish NSD1 orthologs, Nsd1a and Nsd1b, in promoting heterochromatin mediated repression. Our results provide proof-principle that this transgenic reporter line can be used to rapidly identify genes with potential roles in heterochromatic silencing in the context of a live, vertebrate organism.

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