4.3 Article

The complete chloroplast genome of Clematis florida Thunb. (Ranunculaceae), an ornamental and medicinal plant from Henan province, China

Journal

MITOCHONDRIAL DNA PART B-RESOURCES
Volume 7, Issue 3, Pages 471-473

Publisher

TAYLOR & FRANCIS LTD
DOI: 10.1080/23802359.2022.2049460

Keywords

Clematis florida Thunb; chloroplast genome; phylogeny; Ranunculaceae

Funding

  1. Science and Technology Innovation Team of Xinyang Agriculture and Forestry University [CXTD202002]
  2. Experimental Teaching Demonstration Center of Xinyang Agriculture and Forestry University [202102]
  3. Foundation of Central Laboratory of Xinyang Agriculture and Forestry University

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Clematis florida is an ornamental and medicinal plant with great commercial potential. The complete chloroplast genome of C. florida was characterized, providing insights into its phylogenetic relationships and taxonomic status among other Clematis species. This study serves as a reference for future research on molecular biology, evolution, and taxonomy in the genus Clematis.
Clematis florida Thunb. is a herbaceous and perennial plant native to East Asia. The plant is resistant to cold but sensitive to heat. It is an ornamental and medicinal plant that has great commercial potential. Here, we assembled and characterized the complete chloroplast (cp) genome of C. florida. The cp genome of C. florida was characterized by Illumina pair-end sequencing and is 159,606 bp in total length. The genome includes a large single-copy (LSC) region of 79,467 bp, a small single-copy (SSC) region of 18,057 bp, and a pair of inverted repeats (IR) regions of 31,041 bp. The genome contains 135 genes including 91 protein-coding, 36 tRNA, and eight rRNA genes. Phylogenetic analysis based on 18 Clematis species indicates that C. florida is closely related to C. fusca in the Ranunculaceae. The phylogenetic relationships and taxonomic status of C. florida revealed by cp genome were consistent with the previous molecular studies, and can serve as a reference for future studies on molecular biology, evolution, and taxonomy in the genus Clematis.

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