Journal
MITOCHONDRIAL DNA PART B-RESOURCES
Volume 7, Issue 4, Pages 590-592Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/23802359.2022.2057248
Keywords
Delphinium montanum; Pyrenean Larkspur; Ranunculaceae; chloroplast (cp) genome; Illumina sequencing; Nanopore sequencing
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Funding
- interreg-POCTEFA program [FLORALAB Project] [EFA294/19]
- Laboratoires d'Excellences (LABEX) TULIP [ANR-10-LBX-41]
- Ecole Universitaire de Recherche (EUR) TULIP-GS [ANR-18EURE-0019]
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A hybrid assembly approach was used to successfully assemble the complete plastid genome of the endangered Delphinium montanum, which contains 136 genes and supports the polyphyly of the Delphinium genus.
Delphinium montanum DC. 1815, is an endangered larkspur endemic to the Eastern Pyrenees. For biogeographic and conservation purpose, a hybrid assembly approach based on long- and short-read genomic data allowed us to successfully assemble whole plastid genome of Delphinium montanum. The complete plastome is 154,185 bp in length, consisting of a pair of inverted repeats (IRs) of 26,559 bp, a large single-copy (LSC) region and a small single-copy region (SSC) of 84,746 and 16,320 bp, respectively. It was found to contain 136 genes, including 84 protein-coding genes, 44 trRNA genes and 8 rRNA genes. The overall GC content of the plastid genome is 38.3%. Phylogenetic inference supports the polyphyly of the Delphinium genus.
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