Journal
BIOMASS CONVERSION AND BIOREFINERY
Volume -, Issue -, Pages -Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s13399-022-02789-3
Keywords
Flocculation; pH; Fatty acid methyl esters; Biodiesel; Response surface methodology
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Funding
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior -Brasil (CAPES) [001]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [CNPq] [PQ 308063/2019 -8]
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This study investigates the efficiency of Moringa oleifera seeds as a bioflocculant for harvesting Tetradesmus dimorphus biomass. The results show that salt extracts exhibit higher flocculation efficiency than aqueous extracts. Prediction values obtained from data modeling approach real values, allowing for an adequate optimization of flocculant concentration and pH.
Bio-flocculation is a sustainable low-cost harvesting technique for microalgae biomass production; however, it is generally less efficient than chemical flocculants. This study aims to investigate the efficiency of Moringa oleifera seeds as a bioflocculant for harvesting Tetradesmus dimorphus biomass. Four extracts from integral and residual (seeds without lipids) biomass of M. oleifera seeds using salt or aqueous solutions were used at four concentrations (100, 200, 300, and 400 ppm). Flocculation efficiency (FE) increased as the pH decreased. The addition of the extracts reduced the pH of the cultures, dispensing pH adjustment after dosing the flocculating agent. Salt extracts exhibited higher flocculation efficiency than aqueous extracts. The highest flocculation efficiency (similar to 98%) was obtained using a salt extract of residual biomass of seeds in any concentration varying from 100 to 400 ppm. The predicted values obtained from a data modeling using response surface methodology approached the real values (r(2) = 0.9382), resulting in an adequate optimization of the flocculant concentration of similar to 335 ppm and pH 5.6 for a predicted FE of similar to 106%. The findings of the present study confirmed that the salt extract from residual biomass of M. oleifera seeds is an effective bio-flocculant for T dimorphus biomass harvesting.
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