Insecticidal Effect of Pistacia lentiscus (Anacardiaceae) Metabolites against Lobesia botrana (Lepidoptera: Tortricidae)

The extensive use of synthetic insecticides in agriculture poses a great risk for human health and the ecosystem, and mandates the development of safer alternatives derived from natural products. In the present study, we assessed the larvicidal effect of Pistacia lentiscus fruits, leaves, and bark extracts and their components on larvae of a major vine pest, Lobesia botrana. Pistacia lentiscus is an evergreen shrub or small tree possessing significant medicinal value with numerous therapeutic uses since antiquity. Using petri dish residual exposure and topical application bioassays we demonstrated that the fruit extract of P. lentiscus and its metabolites were toxic on L. botrana larvae. Extracts from leaves and bark showed no effect. Bioassay-guided fractionation of P. lentiscus fruit hexane extract led to the identification of its constituents with insecticidal properties on L. botrana larvae. Specifically, we have identified that the main contributor to the bioactivity of the hexane extract of P. lentiscus fruits is its major fraction, PLFHe2 (76.25%). Furthermore, we have found that PLFHe2 is a mixture of triglycerides and that the fatty acids responsible for the observed toxicity are oleic and linoleic acid.

Automated summary

This study demonstrated the larvicidal effect of Pistacia lentiscus fruit extract and its metabolites on Lobesia botrana larvae, with the main contributor to bioactivity being identified as the major fraction PLFHe2, consisting of triglycerides and oleic and linoleic acid as the toxic components.