Journal
FOOD SCIENCE & NUTRITION
Volume 10, Issue 7, Pages 2318-2324Publisher
WILEY
DOI: 10.1002/fsn3.2840
Keywords
(S)-equol; daidzein; recombinant Escherichia coli; whole-cell biotransformation
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Funding
- Natural Science Foundation of Tianjin City [18JCQNJC78900, 18JCQNJC09900]
- National Natural Science Foundation of China [31801471, 31701172]
- Tianjin 131 innovative talent team [201927]
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The study describes the improvement of engineered E. coli for the production of (S)-equol, the most active metabolite of soybean isoflavones. The optimal conditions for (S)-equol production were explored, leading to high yields of (S)-equol.
(S)-equol, the most active metabolite of the soybean isoflavones in vivo, has exhibited various biological activities and clinical benefits. Existing studies on the heterologous biosynthesis of (S)-equol via the engineered E. coli constructed have been significantly progressed. In the present study, the engineered E. coli was further improved to be more suitable for (S)-equol production. The four enzymes involved in the biosynthesis of (S)-equol and another GDH for NADPH regeneration were combined to construct the recombinant E. coli BL21(DE3). The optimal conditions for (S)-equol production were explored, respectively. The yield of equol reached 98.05% with 1 mM substrate daidzein and 4% (wt/vol) glucose. Even when the substrate concentration increased to 1.5 mM, (S)-equol could maintain a high yield of 90.25%. Based on the 100 ml one-pot reaction system, (S)-equol was produced with 223.6 mg/L in 1.5 h. The study presented a more suitable engineered E. coli for the production of (S)-equol.
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