Epidemiological Cut-Off Values and Multidrug Resistance of Escherichia coli Isolated from Domesticated Poultry and Pigs Reared in Mwanza, Tanzania: A Cross-Section Study

Simple Summary The objectives of this study were to determine the prevalence of multidrug resistance phenotypes and the distribution of Escherichia coli among poultry and pigs. Laboratory procedures were conducted according to standard operating procedures and international guidelines. Our findings showed that poultry and pigs reared in Mwanza, Tanzania, are colonized with resistant bacterial phenotypes. Further, different populations of intestinal flora, E. coli, exist between poultry and pigs. Increasing antimicrobial resistance (AMR) colonizing domesticated animals is a global concern threatening food safety. This study aimed at determining the prevalence of multidrug resistance (MDR) and epidemiological cut-off values (ECVs) of E. coli isolated from poultry and pigs in Mwanza, Tanzania. This cross-sectional study was conducted between June and August 2021, involving 297 pigs, 191 broilers, and 203 layers. Rectal and cloacal swabs were collected and processed following standard guidelines. ECVs were determined using normalized resistance interpretation (NRI), a computer software, and descriptive analysis was performed using STATA version 13.0. The overall prevalence of MDR E. coli was 63.2%, whereas poultry (87.5% layers and 86.3% broilers) were more colonized than pigs (31.8%) (p < 0.001). Based on ECVs of antibiotics tested, E. coli from broilers, layers, and pigs exhibited different resistance patterns hence different populations. Exotic breed (p < 0.001) and recent antimicrobial use (p < 0.001) significantly predicted colonization with MDR E. coli. Veterinary officers should implement regulations that prohibit the inappropriate use of antimicrobial agents in livestock keeping.

Automated summary

This study investigated the prevalence of multidrug resistance phenotypes and the distribution of Escherichia coli in poultry and pigs in Mwanza, Tanzania. The results showed that poultry and pigs are commonly colonized by resistant bacterial phenotypes. There are different populations of E. coli between poultry and pigs. The increasing antimicrobial resistance in domesticated animals is a global concern for food safety. The study highlights the importance of implementing regulations to prevent the inappropriate use of antimicrobial agents in livestock keeping.