Preliminary Findings on How Different Management Systems and Social Interactions Influence Fecal Glucocorticoid Metabolites in White Rhinoceros (Ceratotherium simum)

Simple Summary In recent years, interest in improving the welfare of wild species in captivity has grown. The aim of this investigation was to evaluate the effects of different social environments and management systems on the cortisol secretion of fourteen white rhinoceroses (Ceratotherium simum) living under different conditions by using use noninvasive methods. The fecal glucocorticoid metabolite secretion was found to be affected by both management systems and social interactions. Additionally, sex is another factor that seems to influence. This research provides a deeper understanding of glucocorticoid production in white rhinoceroses, but more studies are needed to fully understand its influence on reproductive biology. White rhinoceroses (Ceratotherium simum) are the most social and gregarious species of all rhinoceroses known worldwide. One of the most critical effects of elevated glucocorticoid concentrations, especially in threatened species, is its relation to chronic stress, which could potentially lead to immunosuppression and reduced reproductive activity. Our aim is to determine how different social environments and management systems might be influencing the secretion of fecal glucocorticoids in white rhinoceroses. We have analyzed the concentration of fecal glucocorticoid metabolites in 658 fecal samples from 14 white rhinoceroses, seven free-ranging rhinos, and seven rhinos from two different managed captive populations. HPLC techniques were used to determine the main glucocorticoid metabolite found in this species, and a competitive EIA was used to establish fecal 5 alpha-pregnan-3 3 beta, 11 beta, 21-triol-20-oneglucocorticoid metabolite (fGCM) levels. Our results reveal that management systems and social interactions had an influence on fGCM levels, suggesting that the more restrictive the management and social conditions are, the higher the glucocorticoid concentrations that are found. Additionally, sex was also found to influence fGCM levels, as in females, fGCM concentrations were higher than in males. We conclude that the analysis of glucocorticoids in relation to other factors is a powerful tool to assess adrenocortical response in white rhinoceros in order to broaden the knowledge of their reproductive biology and improve the management of the species.

Automated summary

This study evaluated the effects of different social environments and management systems on cortisol secretion in white rhinoceroses. The results showed that management systems, social interactions, and sex influenced the secretion of fecal glucocorticoid metabolites. This research provides insight into the glucocorticoid production in white rhinoceroses and its impact on reproductive biology.