Replacement of Maize Silage and Soyabean Meal with Mulberry Silage in the Diet of Hu Lambs on Growth, Gastrointestinal Tissue Morphology, Rumen Fermentation Parameters and Microbial Diversity

Simple Summary A shortage of high-quality roughage jeopardises the Chinese mutton sheep industry. The development of new roughage resources is important to safeguard the health and welfare of the sheep, to save costs, increase efficiency and improve resource utilization. Mulberry leaves have high nutritional value and have been used in herbivore production for a long time in China. However, fresh mulberry leaves are not easy to preserve, and dried mulberry leaves readily lose nutrients in the conservation process. Ensiling mulberry leaves can reduce the anti-nutritional constituents, mainly phytic acid and tannin, while reducing any nutrient loss. In this study, mulberry silage replaced part of a maize silage-based diet for fattening Hu lambs. The effects of mulberry silage on the growth of the lambs, their gastrointestinal tissue morphology, rumen fermentation parameters and bacterial diversity were investigated. The results showed that using mulberry silage in place of 20-40% of the maize silage in the diet of Hu lambs promoted their growth, while maintaining satisfactory digestion. Maize silage has a significant environmental impact on livestock due to its high requirement for fertilizer and water. Mulberry has the potential to replace much of the large amount of maize silage grown in China, but its feeding value in the conserved form needs to be evaluated. We fed Hu lambs diets with 20-60% of the maize silage replaced by mulberry silage, adjusting the soybean meal content when increasing the mulberry silage inclusion rate in an attempt to balance the crude protein content of the diets. Mulberry silage had higher crude protein and lower acidic and neutral detergent fiber contents compared to maize silage. Replacing maize silage and soyabean meal with mulberry silage had no effect on the feed intake and growth rate of Hu lambs. However, the rumen pH increased, the acetate to propionate in rumen fluid increased, and the rumen ammonia concentration decreased as mulberry replaced maize silage and soyabean meal. This was associated with an increase in norank_f__F082 bacteria in the rumen. Rumen papillae were shorter when mulberry silage replaced maize silage, which may reflect the reduced neutral detergent fiber (NDF) content of the original silage. In conclusion, mulberry silage can successfully replace maize silage and soyabeans in the diet of Hu lambs without loss of production potential, which could have significant environmental benefits.

Automated summary

A shortage of high-quality roughage jeopardises the Chinese mutton sheep industry. The development of new roughage resources is important to safeguard the health and welfare of the sheep, to save costs, increase efficiency and improve resource utilization. The study found that using mulberry silage in the diet of Hu lambs promoted their growth and maintained satisfactory digestion. Replacing maize silage and soybean meal with mulberry silage had no effect on the feed intake and growth rate of Hu lambs. Rumen fermentation parameters and bacterial diversity were also affected by mulberry silage inclusion. This research is important for improving sheep feed formulation, enhancing resource utilization, and reducing environmental impact.