Analysis of Intestinal Microbial Diversity of Four Species of Grasshoppers and Determination of Cellulose Digestibility

Simple Summary Grasshoppers are typical phytophagous pests, which prefer eating monocotyledons with more cellulose and hemicellulose. Due to its large appetite and high utilization rate, the intestinal contents of grasshoppers have the potential to be developed into a bioreactor, which can be applied to improve straw utilization efficiency in the future. The digestive tract of grasshoppers is a complex ecosystem, inhabited by a large number of microorganisms. The existence of these microorganisms enables grasshoppers to have high decomposition and utilization of plant fibers. However, there are few reports on the microflora structure and diversity of the digestive tract of grasshoppers. In this study, the diversity of symbiotic bacteria in the intestinal tract of four species of grasshoppers, namely Acrida cinerea, Trilophidia annulata, Atractomorpha sinensis and Sphingonotus mongolicus, was studied by using the method of constructing a 16S rRNA gene library and Illumina Miseq sequencing technology. At the same time, the digestibility of cellulose and hemicellulose of the four species of grasshoppers were determined and the relationship between digestibility and intestinal microbial diversity was analyzed. This study provided basic data for the development of the digestible bioreactor of cellulose and hemicellulose, which may provide a new idea for degrading straw. Grasshoppers (Insecta, Orthoptera, Acridoidea) are a large group of agricultural and animal husbandry pests. They have a large food intake with high utilization of plants fibers. However, the composition of the grasshopper gut microbial community, especially the relationship between gut microbial community and cellulose digestibility, remains unclear. In this research, 16S rRNA gene sequences were used to determine the intestinal microbial diversity of Acrida cinerea, Trilophidia annulata, Atractomorpha sinensis and Sphingonotus mongolicus, and Spearman correlation analysis was performed between the intestinal microbes of grasshoppers and the digestibility of cellulose and hemicellulose. The results showed that Proteobacteria was the dominant phylum and Klebsiella was the dominant genus in the guts of the four species of grasshoppers; there was no significant difference in the species composition of the gut microbes of the four species of grasshoppers. Spearman correlation analysis showed that Brevibacterium and Stenotrophomonas were significantly correlated with cellulose digestibility. Brevibacterium, Clavibacter, Microbacterium and Stenotrophomonas were significantly associated with hemicellulose digestibility. Our results confirmed that the gut microbes of grasshoppers were correlated with the digestibility of cellulose and hemicellulose, and indicated that grasshoppers may have the potential to develop into bioreactors, which can be applied to improve straw utilization efficiency in the future.

Automated summary

This study investigated the diversity of symbiotic bacteria in the intestinal tract of four grasshopper species and analyzed the relationship between intestinal microbial diversity and the digestibility of cellulose and hemicellulose. The results showed that gut microbes were correlated with the digestibility of cellulose and hemicellulose, suggesting the potential of grasshoppers to develop into bioreactors for improving straw utilization efficiency.