Application of the Integrated Supercritical Fluid Extraction-Impregnation Process (SFE-SSI) for Development of Materials with Antiviral Properties

The integrated supercritical fluid extraction-impregnation process (SFE-SSI) was performed to fabricate material with antiviral properties against the herpes simplex virus (HSV). Cotton gauze and starch/chitosan polymer films (SCF) were impregnated with components extracted from Melissa officinalis at 10 MPa and 40 degrees C using a green medium, supercritical carbon dioxide (scCO(2)). The influences of the processing mode regarding the flow of the supercritical fluid through the system, and the mass ratio of the plant material and the solid carrier, on the impregnation yield of M. officinalis extract were studied. The results revealed that the introduction of a fresh amount of CO2 into the system enabled the highest impregnation yield of 2.24% for cotton gauze and 8.71% for SCF. The presence of M. officinalis extract on the surface of both impregnated cotton gaze and SCF was confirmed by FTIR and GC analyses after the re-extraction of the impregnated samples. The M. officinalis impregnated materials showed a strong inhibitory effect against Bovine herpesvirus type 1 (BHV-1).

Automated summary

The integrated supercritical fluid extraction-impregnation process was used to fabricate materials with antiviral properties against herpes simplex virus (HSV) by impregnating cotton gauze and starch/chitosan polymer films with Melissa officinalis extract. The study investigated the effects of the processing mode and the mass ratio of plant material to solid carrier on the impregnation yield. The impregnated materials showed a strong inhibitory effect against Bovine herpesvirus type 1 (BHV-1).