Journal
FOOD PACKAGING AND SHELF LIFE
Volume 31, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.fpsl.2021.100786
Keywords
Active packaging film; Non-migrating active packaging; Covalent immobilization; Antimicrobial
Categories
Funding
- National Key R&D Program of China [2020YFD0900905]
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Non-migrating active antibacterial packaging, prepared by immobilizing chitosan or lysozyme on plasma-treated polylactic acid film, showed inhibitory effects on E. coli and S. aureus survival. The grafted films demonstrated promise in providing antibacterial effects for fresh fish packaging.
Non-migrating active antibacterial packaging can provide long-term and safe effects on food preservation. It was prepared by covalently immobilizing chitosan (CS) or lysozyme (LYS) on the surface of plasma-treated polylactic acid (PLA) film. Films were characterized in terms of X-ray photoelectron spectroscopy (XPS), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), field emission scanning electron microscopy (FESEM). Laser scanning confocal microscopy (LSCM) revealed that survival of E. coli and S. aureus was inhibited on CS or LYS functionalized films. Finally, the grass carp fillets were stored at 4 degrees C for 9 days, and physicochemical changes and microbiological counts were determined. Results showed that CS grafted PLA film (CS-gPLA) and LYS grafted PLA film (LYS-g-PLA) retarded the decay of fish fillets for 1 and 3 days, respectively, compared to control sample. The CS-g-PLA film inhibited the growth of bacteria more efficiently than PLA and LYS-g-PLA films (P < 0.05). Overall, this study demonstrated that grafted films are a promising non-migrating packaging material for providing antibacterial effects in packaging for fresh fish.
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