Journal
PHARMACEUTICS
Volume 14, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/pharmaceutics14051008
Keywords
I-123-BMIPP; SPECT; nuclear medicine imaging; bacterial infection; bacterial imaging
Categories
Funding
- Japan Society for the Promotion of Science [18K19508, 18K07747, 21H02865]
- SHIONOGI Co., Ltd.
- Grants-in-Aid for Scientific Research [18K19508, 18K07747, 21H02865] Funding Source: KAKEN
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This study evaluated the use of I-123-BMIPP to visualize fatty acid metabolism in bacteria for bacterial infection imaging. The results showed that I-123-BMIPP accumulated in Escherichia coli EC-14 and the uptake was mediated by a fatty acid transporting membrane protein. In infected mice, I-123-BMIPP showed higher contrast in infected muscle compared to non-infected muscle, indicating its potential for imaging bacterial infections.
In this study, we evaluated the use of 15-(4-I-123-iodophenyl)-3(R,S)-methylpentadecanoic acid (I-123-BMIPP) to visualize fatty acid metabolism in bacteria for bacterial infection imaging. We found that I-123-BMIPP, which is used for fatty acid metabolism scintigraphy in Japan, accumulated markedly in Escherichia coli EC-14 similar to F-18-FDG, which has previously been studied for bacterial imaging. To elucidate the underlying mechanism, we evaluated changes in I-123-BMIPP accumulation under low-temperature conditions and in the presence of a CD36 inhibitor. The uptake of I-123-BMIPP by EC-14 was mediated via the CD36-like fatty-acid-transporting membrane protein and accumulated by fatty acid metabolism. In model mice infected with EC-14, the biological distribution and whole-body imaging were assessed using I-123-BMIPP and F-18-FDG. The I-123-BMIPP biodistribution study showed that, 8 h after infection, the ratio of I-123-BMIPP accumulated in infected muscle to that in control muscle was 1.31 at 60 min after I-123-BMIPP injection. In whole-body imaging 1.5 h after I-123-BMIPP administration and 9.5 h after infection, infected muscle exhibited a 1.33-times higher contrast than non-infected muscle. Thus, I-123-BMIPP shows potential for visualizing fatty acid metabolism of bacteria for imaging bacterial infections.
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